Comparison of the identification of Acinetobacter spp. with API20NE and 16S rRNA gene sequencing techniques

Acinetobacter species are aerobic, encapsulated, oxidase-negative, non-motile, non-fermentative Gram-negative coccobacilli. Until recently, the genus Acinetobacter contained the single species A. caicoaccticus, which was subdivided into two subspecies or biovars (A. calcoaceticus subspecies anitratus and A. calcoaceticus subspecies Iwoffii). In 1986, the taxonomy of the genus Acinetobacter was altered extensively by Bouvet and Grimont,1 who outlined 12 different species by DNA-DNA hybridisation, including the named species A. baumannii, A. calcoaceticus, A. haemolyticuS, A. johnsonii, A. jUnii, and A. Iwoffii, and six unnamed genomic species. More recently, 16S rRNA gene sequence analysis …